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Heliyon 10(7):e28093 Hu Y et al (2024) Emerging roles of ferroptosis in pulmonary fibrosis: current perspectives, opportunities and challenges

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ERR is expressed in multiple human breast cancer cell lines, including ZR75.1, MDA-MB-231, HS587T, BT549, SK-BR-3, T47D, MCF-7, MDA-MB-448, and BT474 ( In vitro experiments using human breast adenocarcinoma SK-BR-3 and human breast tumor fibroblast cell line WS3TF showed that ERR increased the expression of CYP19A1 by binding to a silencer element (i.e., a type of regulatory DNA sequence) named S1, which allowed for promoter I.3 to increase CYP19A1 transcription, resulting in increased activity ( Figures 2A , 3 )

dissociation buffer 2: 10 mM TrisHCl, 0.1% BSA, 5 mM EDTA pH 7.4