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Nutrients, 16, 3665

Moreover, it has been reported that intervals of 3060 min of physical activity are when BDNF is reliably elevated and antecedent to physical activity segmenting to 90 min or more indicates that counterregulatory mechanisms would be activated resulting in cortisol release that likely suppresses BDNF expression [209]

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After 24 h, viability was assessed using the AquaBluer assay as previously described ( Determination of GSH Levels HT-1080 cells were cultured in DMEM containing 10% FBS, 1% supplemented non-essential amino acids and 1% Pen-Strep mixture (all from Gibco), and maintained in a humidified atmosphere with 5% CO 2 at 37C