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This is supported by metabolomic profiling studies that have identified complex lipid species and amino acid analogues associated with the response to weight loss interventions

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So in answer to your question - 'is there any point in testing for iron when supplemention has taken place - well, yes (for the reasons above) especially if supplementation is sporadic

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That same research also found that infertile men were more likely than fertile men to have low levels of vitamin C in their semen