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Jurkat T cells were grown at 37 C in 5% CO 2 -humidified air in RPMI medium that contained 10% fetal bovine serum (FBS), 100 U/ml penicillin and 100 mg/ml streptomycin

Almost all AD patients exhibit erratic sleep patterns, nocturnal staying awake, and daytime sleepiness (Spira et al., 2013)

doi: 10.1186/1471-2407-13-299 24 DuXJLiXMCaiLBSunJCWangSYWangXCet al

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The wells were washed three times with PBS-T to remove unbound secondary antibody