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To confirm that H 2 O 2 increased ROS, we used DCFH-DA to measure ROS and observed that it was significantly increased 4 h after treatment (Fig

Finally, GSH depletion was designated as necessary to induce the redox unbalance characteristic of the induction phase of the mitochondrial pathway but not sufficient per se to assure the execution phase of apoptosis

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Vitamin C helps convert oxidized glutathione back into its active form This regeneration process allows glutathione to continue working efficiently Supports sustained antioxidant activity rather than short-term effects Without Vitamin C, glutathione can lose its effectiveness more quickly

Jalewa et al., 2017
