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If your GST-fusion protein appears to be unstable, or denatured, in the elution buffer, you may want to check if glutathione is the source of this instability

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10.1016/S0022-3476(99)70121-6
Lysate was clarified by centrifugation (21,000 g , 30 min, 4 C in case of PBS, r.t

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