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Regenerates other antioxidants: Glutathione recycles vitamin C and vitamin E back to their active forms after they've neutralized free radicals so other antioxidants depend on adequate glutathione to keep working

Contact time for cleaning solutions Cleaning Solution Contact Time 1.5 M NaCl 15-20 minutes 1 M NaOH 1-2 hours (not overnight) 30% isopropanol 15-20 minutes 70% ethanol 15-20 minutes 0.5% non-ionic detergent in 0.1 M acetic acid 1-2 hours (not overnight) Once you are done washing with these buffers, you will immediately rinse with a few column volumes of dIH2O followed by a few column volumes with binding buffer, or some kind of buffer with a neutral pH around 7 to 8