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After being fed HFD for a total of 16-weeks (an appropriate rodent model of DIO and comparable to human obesity [21]) and reaching pre-arrival body weights (~38g), mice were randomized into balanced control and treatment cohorts (n=9/cohort), with similar group average body weight and standard deviation

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The microorganisms in the biofilm include heterotrophic bacteria, fungi, and protozoa, each contributing to the breakdown of different types of organic matter: Bacteria primarily degrade dissolved organic carbon (DOC), such as carbohydrates and proteins