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After obtaining 70 % cell confluency (about 24 h culturing incubation time)

This enzyme is encoded by a single gene in Arabidopsis (At5g43940), and catalyzes the NADH-dependent reduction of GSNO to S -aminoglutathione, which then decomposes to ammonia and free GSH (Sakamoto et al., 2002

10.1104/pp.102.4.1193 109 MayoS.CominelliE.SparvoliF.Gonzlez-LpezO.Rodrguez-GonzlezA.GutirrezS.et al

Giannakou ME, Partridge L

When considering supplementation, the form of glutathione is critical

Nevertheless, drawbacks of EDEA and BDEA, such as high hydrophobicity and low solubility, as well as the strong binding to serum albumin, need to be solved in future structural optimization to obtain better membrane permeability and solubility [204]