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On the day of discharge, her anion gap had risen to 29 mEq/L

Electrophoretic mobility shift assay Total protein was extracted using Cell lysis buffer for Western blotting and immunoprecipitation (Beyotime, Shanghai)

[DOI] [PubMed] [Google Scholar] 16.Hanif F., Muzaffar K., Perveen K., Malhi S.M., Simjee S.U

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Notably, a small cell lung carcinoma cell line, which expresses POMC and ACTH, possesses a hypomethylated POMC promoter, suggesting that ectopic ACTH secretion by tumors may be due to hypomethylation at a relatively early stage in carcinogenesis (41)
