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In recent times, several investigations have been seeking an explanation for the great variability in both outcome and toxicity in cisplatin-based therapy th...

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For solubilizing these polypeptides, variables such as non-denaturing detergents (IGEPAL CA-630), changing the ionic strength of the cell lysis and solubilization buffer, addition of BugBuster, diluting the cell lysate and sonication were introduced