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Subsequently, according to the method described in the previous study ( 2.7 Immunofluorescence (IF) After transcardial perfusion in mice, brain tissues were harvested and pretreated with gradient dehydration in 20% and 30% sucrose solutions at 4C (to reduce ice crystal damage), then embedded in optimal cutting temperature compound and sectioned for 20 m thickness using cryostat

Statistical analysis The peak areas of each detected VOC (arbitrary units) and the concentration of each SCFA (mM) were assessed as quantitative variables

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This method, like DTI, can be performed as an in-life assessment, offering valuable information on the evolution of myelin changes

Maximum dose (Adults): 15 mg subcutaneously once a week