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Chemicals We obtained from Sigma-Aldrich (Madrid, Spain) synthetic lipids (also from Avanti Polar Lipids Inc., Alabaster, AL, USA), fatty acid methyl ester standards (also from Larodan Fine Chemicals, Mlmo, Sweden), methyl tert-butyl ether (MTBE) liquid chromatography coupled to mass spectrometry (LC-MS), acetonitrile LC-MS, isopropanol LC-MS, potassium chloride, chloroform, ammonium formate and ammonium hydroxide

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In these conditions, BBB disruption leads to increased permeability, which facilitates neuroinflammation, exacerbates neuronal damage, and accelerates disease progression