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After organelle removal by centrifugation at 7800 g for 5 min, the sample was centrifuged at 16,000 g for 30 min to obtain the supernatant containing vesicle proteins and the pellet containing PM proteins

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Treatment of long-range electrostatic interactions was set to Particle Mesh-Ewald (PME) 61 , and the short-range electrostatic and van der Waals cut-off set to 1.0 nm

A survival pathway for Caenorhabditis elegans with a blocked unfolded protein response