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To understand the underlying molecular mechanisms, the expression levels of 3-AR mRNA in both white and brown adipose tissue were assessed using reverse transcription followed by PCR and Southern blotting

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Retatrutide's Phase 2 trial data (Jastreboff et al., NEJM 2023) identified a side-effect profile broadly consistent with other incretin-based therapies: Gastrointestinal : Nausea, vomiting, diarrhoea, and constipation were the most commonly reported adverse effects, particularly during dose escalation