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Hashiba K., Taguchi M., Sakamoto S., et al., Overcoming Thermostability Challenges in mRNALipid Nanoparticle Systems With PiperidineBased Ionizable Lipids, Communications Biology 7, no

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doi: 10.1007/s11011-022-00929-2 114

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The following day, Ni-NTA resin equilibrated in buffer A was added over the cleaved laccase samples and incubated at 25 C and 1,000 rpm in a thermomixer comfort shaker (Eppendorf) for 4 h