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Images were analyzed using the Adiposoft plug-in software in ImageJ (NIH).[22] Briefly, images were converted to 8-bit images and scald to 0.366 microns per pixel (corresponding to 20 magnification on the Leica microscope)

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This can manifest as brain fog, difficulty concentrating, and difficulty completing tasks

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In Figure 4, vitamin B12 level was significantly decreased in rats treated with PTU (297.52 12.51) compared with the control group (443.11 22.54)