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After euthanasia, the pancreas was perfused via the common bile duct with 24 mL of digestion buffer (Hanks Balanced Salt Solution containing type IV collagenase, 1 mg/mL, Gibco, 17104019)

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In fact, reports of bacterial endotoxin in the pelvic cavity and menstrual blood of people with endometriosis 37 have led to a bacterial contamination hypothesis suggesting endotoxin/bacteria in the menstrual effluent contributes to pelvic inflammation, growth, and progression of endometriotic lesions (reviewed in 38,39 )

G., Linkova, N

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The clarified liquid obtained was vortexed until the volume of the liquid stabilized and did not decrease any more