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Immunohistochemical staining by an automated immunostainer (Dako Autostainer Plus, DakoCytomation, Glostrup, Denmark) was performed using LSAB HRP and HRP+ kits according to the manufacturers instructions
(Czechia) using validated chromatographic, spectrometric and mass spectrometry techniques

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doi: 10.1080/15360288.2022.2084488