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The absorbance of the reaction product was immediately measured at 412 nm using a Perkin Elmer lambda 20 spectrophotometer

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However, the effect of oxidative and nitrosative stress is not restricted to the intracellular environment, as reactive species can cross cellular membranes and influence extracellular proteins

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[DOI] [PubMed] [Google Scholar] Lunetta KL, D'Agostino RB, Karasik D, Benjamin EJ, Guo CY, Govindaraju R, Kiel DP, Kelly-Hayes M, Massaro JM, Pencina MJ, Seshadri S, Murabito JM