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Epithalon + Thymalin Investigates combined pinealthymic signaling in immunosenescence research

Buffered solutions are generally not used for primary reconstitution because freezethaw cycling of buffer salts can cause local pH shifts during freezing

Real-time quantitative PCR (RT-qPCR) The total RNA was extracted from leukocytes using the QIAamp RNA Blood Kit (Qiagen, Hilden, Germany), and cDNA was produced with the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher, Waltham, MA) as previously described 18