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For the deacetylation assay, purified SIRT7 protein was incubated with acetylated SRF peptide in deacetylation buffer (50 mM TrisHCl pH 8.0, 4 mM MgCl 2 , 0.2 mM DTT, 1 mM NAD + and 1 protease inhibitor cocktail) at 37 C for 1 h

In contrast, most PAGs are at least twofold downregulated in both 35S::SLIM1 lines relative to Col-0 at 44 DAS ( Figure 3C , Table 1 , Supplementary Table S1 )

doi: 10.1160/TH16-08-0593 41 NaitoYTakagiTHigashimuraY

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Crowe et al

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