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The transfection protocol involved seeding NPCs in 6-well plates to 6070% confluence, then incubating the cells for 6 h with a transfection complex comprising 50 nM siRNA, Opti-MEM, and Lipofectamine 2,000 followed by replacing the medium with growth medium and culturing for 72 h

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The shortening of the telomeresthe protective DNA sequences at the ends of chromosomeshappens as we get older, and this phenomenon constitutes a hallmark of aging

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