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Primary cells and immortalized cell lines are generally cultured under atmospheric oxygen levels, which are higher than the physiological oxygen content in vivo , leading to a pro-oxidative environment in vitro ( in vitro without specific stimulus inducing oxidative stress

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Scan J Gastroenterol

Vitamin D affects Nrf2 levels (p-value = 0.08) and HDAC2 (p-value = 0.01)

The slides were incubated with fluorescently labeled secondary antibodies (Alexa Fluor 488-conjugated goat anti-mice immunoglobulin G, Beyotime, China or Alexa Fluor 488-conjugated goat anti-mice immunoglobulin G, Beyotime, China) for 2 h at 37C and 4,6-diamidino-2-phenylindole (DAPI) (#c-1002, Beijing, China) for half an hour at room temperature, and finally, the slides were sealed with antifluorescent quencher and nail polish

Basuli, D