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After stimulation, samples were lysed with RIPA and stored at 80 C

Therefore, ALA has been suggested as an optimal and promising antioxidant, metal chelator, ROS scavenger and organizer for other antioxidants under stress conditions (Navari-Izzo et al., 2002)

doi: 10.1016/j.ebiom.2024.105217 89 HeathKEFeduskaJMTaylorJPHoupJABottaDLundFEet al

Subsequent work by Pickart and Margolina identified clusters of affected genes spanning DNA repair, antioxidant defense, ubiquitinproteasome activity, anti-inflammatory signaling, and tissue remodeling [2]

For this reason, various methods have been developed to assess it, methods that are useful in disease research, highlighting either the degree of damage (oxidation in DNA, proteins, lipids) or the ability of the body to self-regulate and also self-defense (through feedback mechanisms, antibodies, etc.) (1,2)