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Nutrients 2017, 9:429
In order to visualize biocytin-filled neurons, brain slices were washed (5 min at RT x 3 times) in PBS and incubated (overnight at 4C) with streptavidin-Alexa 488 (ThermoFisher Scientific, S-11223, 1:500) in PBS-triton 0.05%
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Skin sensations such as itching or flushing
