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Wang M, Yin C, Wang L, Liu Y, Li H, Li M, Yi X, Xiao Y (2019) Serum asprosin concentrations are increased and associated with insulin resistance in children with obesity

Relative level of ROS was detected centrifuging liver tissue in the appropriate buffer (20 mmol/L Tir-HCl (pH 7.4), 20 mmol/L NaH 2 PO 4 , 5 mmol/L MgCl 2 , 130 mmol/L KCl and 30 mmol/L glucose) and incubating the supernatant with DCFH-DA (2, 7-dichlorodihydrofluorescein diacetate) for 15 min at 37 C

[DOI] [PMC free article] [PubMed] [Google Scholar] 307

Fernndez-de-Las-Peas C, Palacios-Cea D, Gmez-Mayordomo V, et al

doi: 10.1016/j.peptides.2020.170369