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Other approaches to establish organoids consist on culturing cell aggregates in suspension or by air-liquid-interface (ALI), another method where the basal cell layers grown in contact with the culture medium, while the external layers are exposed to the air allowing a higher oxygen supply than submerged organoid cultures [161]

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This phenomenon, observed in vitro by Pedroarena and Llins (1997), was identified as an important electrical signature suggestive of pacemaker-like properties

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Surface plasmon resonance studies have revealed a 1mM affinity for PIP 2 within lipid bilayers

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[16] [17] An event like a sunburn, exposure to a chemical, stress or emotional distress can trigger or exacerbate the condition

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