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During the course of the study, there was no change to the therapeutic regime other than the introduction of parenteral cobalamin supplementation

Indeed, the levels of several tight junction proteins, measured by Western blots, including occludin and ZO-1 were decreased in hypertensive models in some studies [92, 94], but not others, where differences in colonic occludin or ZO-1 between hypertensive and normotensive animals was not detected when these were assessed by immunofluorescence [93]

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A significant reduction in appetite and food intake was also observed with tirzepatide compared to placebo in people with T2D [45]