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Group comparisons were performed using one-way or two-way ANOVA followed by Tukeys post hoc test for multiple comparisons

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Cell proliferation assay Cells were seeded in 96-well plates at a density of 30004000 cells per well and allowed to adhere overnight

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At hormonal levels, Copper Tripeptide-1 acts as a chemoattractant for fibroblasts (repairing cells) and activates a plethora of regenerative processes in the skin, such as angiogenesis, production of extracellular matrix components, including collagen, elastin, proteoglycans, glycosaminoglycans, etc, additionally activating metalloproteinases (MMPs) involved in tissue remodeling
