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EPM was performed 0.5 h after ethanol administration, and the samples were pre-administered 0.5 h before ethanol administration

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10.1080/15287390701738467 228 YangW.HaoW.MengZ.DingS.LiX.ZhangT.et al

This step removes positively charged trypsinized peptides from the sample, while negatively charged peptides (e.g., phosphopeptides) are collected in the early eluting fractions

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