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Mitochondrial function in vivo evaluated by NADH fluorescence: from animal models to human studies

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10.1111/1759-7714.12783 168 UehiroN.SatoF.PuF.TanakaS.KawashimaM.KawaguchiK.et al (2016)

Excess DTT and imidazole were removed on a PD-10 desalting column (Merck), and the reduced proteins were eluted with 3.5 mL ice-cold assay buffer containing 100 mM Tris/HCl, 1 mM EDTA, pH 8.0

Better is subjective, and even most larger studies have not been able to really differentiate which one is "better." It will depend on how your body responds to each medication to find out which one is better for you

Validating a chemical series : Small-molecule pull-down with competition elution and PRM/MRM confirmation