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Eligible studies evaluated pharmacological or microbiota-based interventions aimed at ameliorate ASD-relevant outcomes, compared to vehicle, untreated ASD controls, or wild-type/sham controls

Many patients experience softer stools or less frequent bowel movements, which are different from clinical diarrhea (three or more loose stools per day)

For simultaneous GSH and GSSG measurement: HPLC-MS/MS or HPLC with fluorescence detection offers the specificity needed to resolve both species in a single run

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BSO is known for its potential to deplete intracellular GSH

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