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It is produced by the liver and involved in many body processes

Sci Hortic 269:109397

Specifically, within the reproduction-related group, 8 proteins were associated with fertilization or sperm-oocyte interaction (42%

Aug 1981;117(8):465-468

TET1 then further oxidizes 5hmC to 5-formylcytosine (5fC), and subsequently, which is subsequently oxidized to 5-carboxylcytosine (5caC) (43)

Then pellets were resuspended in lysis buffer (Tris-HCl 100 mM and 0.5% Triton X-100, pH 8) containing Halt Protease and Phosphatase Inhibitor cocktail (Thermo Scientific, 1861280), thoroughly vortexed for 1 min and incubated for 15 min on ice