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Pellets were resuspended in 40 L lysis buffer (TBS containing 0.7% triton-X, 0.1% tween-20, and 1 Halt Protease and Phosphatase Inhibitor (PPI) Cocktail (ThermoScientific 78440)) and lysed on ice for 20 min

John Wiley & Sons, 1948

The actual current output is affected by factors such as the battery's internal resistance (converted to heat) and the resistance from the external wires and connector connected to the battery terminals