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The intensity of signals was analyzed by densitometric scanning, using the digital imaging analysis system with AlphaEase Stand Alone Software (Alpha Innotech Corp., San Leandro, CA)

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Moraes JA, Encarnao C, Franco VA, Xavier Botelho LG, Rodrigues GP, Ramos-Andrade I, Barja-Fidalgo C, Renovato-Martins M (2021) Adipose tissue-derived extracellular vesicles and the tumor microenvironment: revisiting the hallmarks of cancer