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G6PD-Deficiency in Monocytes and Macrophages Parsanathan et al

The junction between the oxidation and reduction regions is where calmodulin (CaM) binds

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GPX4 inhibitors GPX4 is a selenase that utilizes GSH as a cofactor to reduce membrane phospholipid peroxide to maintain cellular redox homeostasis, thus negatively regulating ferroptosis

doi: 10.3390/ijms18020248 50 LinY.CradickT

Cell pellets were resuspended in 500 l of IP buffer 1 [50 mM HEPES pH 7.6, 125 mM KCl, 0.1% NP-40, 20% glycerol, 1 mM EDTA, 1 mM PMSF, and Complete protease inhibitor cocktail (Sigma)] and disrupted using Mini-Beadbeater-16 (BioSpec Products)