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Red cell DAMPs and inflammation

Reich, H

Glutathion ist eines der wichtigsten Antioxidantien im Gehirn

Neutrophil apoptosis was determined by flow cytometry after staining Percoll gradient-enriched leukocytes with 10 g/ml anti-CD16/CD32 mAb, 4 g/ml of APC-labeled anti-mouse CD45.2 mAb, 0.5 g/ml of Brilliant violet 421-labeled anti-mouse Ly6G, as well as Alexa Flour 488-labeled annexin V and propidium iodide (PI) using an Alexa Fluor 488 Annexin V/Dead Cell Apoptosis kit (Invitrogen)

These findings led to the use of toxicogenomic profiles for analysis of the cellular responses to OTA

Following this, the column was washed with five column volumes of buffer A and then with 510 column volumes of wash buffer