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Mechanistically, PA reduces the expression of GPX4 and heat shock factor 1 and regulates the transcription of genes related to iron metabolism (such as FTH, TFR1, and SLC40A1), causing iron homeostasis imbalance, while GPX4 overexpression reverses PA-induced ferroptosis in cardiomyocytes

After BSO treatment compared to untreated cells, 296 proteins exhibited significant alterations in abundance (p-value 1.5) (Fig 1b,c

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Acquired resistance refers to the phenomenon that initial tumor cells are sensitive to chemotherapy drugs, but the curative effect of drugs reduces gradually and results in drug resistance after induction therapy

The molecular mass of TTR was independently confirmed by time-of-flight liquid chromatography-mass spectrometry (LC/MS TOF 6230B, Agilent, Santa Clara, CA, USA)