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TAC-2 and MDCK cells were maintained in DMEM supplemented with 10% FBS

The culture media was substituted by the media supplemented with 10% of Cell Count Reagent SF and incubated for 1 h

For a long time, cobalamin has only been known as a widespread organometallic cofactor to many thermally-driven enzymes that catalyse a wide range of processes essential to living organisms, including humans 3
