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(PubMed) Clarke JD, Hsu A, Riedl K, et al

J Clin Invest (2017) 127(7):2492504

Specifically, cellular autophagy markers (LC3-II, SQSTM1/p62) peak in the early morning hours during fasted states, creating an optimal window for FOXO4-DRI absorption and p53 pathway engagement

The absorbance difference at 400 nm was plotted as a function of haem concentration, and the dissociation constant ( K d ) was calculated using a quadratic binding equation