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The safety and dosage of antioxidants are also factors to be considered, as excess antioxidants may have negative effects, such as inhibiting endogenous antioxidant defense systems, increasing oxidative stress, and interfering with the action of other drugs

This parental line, which intrinsically expresses minimal CFTR protein, serves as a reliable model and is frequently used as a null-background for the stable overexpression of wild-type, F508del, or other CFTR variants, enabling the study of their function and response to modulators (Ousingsawat et al., 2011

Its conformation in aqueous systems allows for reproducibility in structure-function evaluations and phospho-peptide comparison studies

Ozone treats spinal pain through its unique effects.11 Ozone can be administered through intradiscal, periganglionic, or paravertebral intramuscular injection.108 After injection, ROS react with the intervertebral disc and shrink it to reduce compression on the spinal cord.109 Other mechanisms may be related to muscle relaxation and analgesic and anti-inflammatory effects.11,108 A randomized control study reported that intramuscular injection of ozone at a concentration of 20 g/mL three times a week for 5 consecutive weeks resulted in improved relief of radiating pain and acute low back pain in lumbar disc herniation patients.108 Another noninferiority randomized control study involving 49 patients with single-level disc herniation reported that intradiscal oxygen-ozone was not inferior to microdiscectomy for lumbar disc herniation.110 However, Roln et al.111 reported that a patient appeared to have an untoward severe reaction after receiving paravertebral ozone therapy and autohemotherapy for spondylarthrosis