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BME-established organoids from different regions of the gastrointestinal tract also rely on integrin 1 We then tested whether the observed integrin 1-regulated proliferation of intestinal organoids in BME also held for organoids derived from other regions of the human gastrointestinal tract: duodenum ( N = 2), J2 jejunum ( N = 2) and N39 ileum ( N = 2), stomach organoids 31 ( N = 1) and ductal-type organoids from the pancreas 32 ( N = 2) and liver 33 ( N = 2)

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doi: 10.1210/en.2006-0242 85 OlsenJMSatoMDallnerOSSandstrmALPisaniDFChambardJ-Cet al

Confocal microscopy Confocal images were acquired using a Leica TCS-SP2 confocal laser scanning unit equipped with argon and helium-neon laser beams and attached to a Leica DM1RB inverted microscope ( Leica Microsystems, Mannheim, Germany)