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Azithromycin may also affect blood thinners (like warfarin), digoxin, and some diabetes or asthma medications

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Preparing your glutathione beads Loading your GST-fusion protein onto the glutathione beads Adding the interacting proteins Eluting the GST-fusion and interacting proteins Materials needed for GST pull-down assays Preparing glutathione agarose beads Loading GST-fusion proteins onto glutathione beads Adding interacting proteins Eluting the beads Analyzing pull-down results What is a GST pull-down assay

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Make sure to only use lukewarm distilled, micro-filtered, bottled or previously boiled and cooled water

Agarose is activated (top right) creating an epoxy reactive group to attach IDA which binds to Ni 2+
