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Cell pellets were resuspended in reducing lysis buffer (20 mM potassium phosphate, 200 mM NaCl, pH 7.4, 10 mM DTT, 0.01 mg ml 1 DNase I) to an OD 600 of 140 and lysed by two rounds of sonication for 2 min at 50% amplitude with 2 s intervals using a Sonopuls (Bandelin) sonicator
Kaneko E, Sato N, Sugawara T, Noto A, Takahashi K, Makino K, et al
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These liposomes: Protect glutathione from gastric acid the lipid shell resists acid degradation far better than the glutathione molecule itself Fuse with intestinal cell membranes releasing glutathione directly inside cells rather than relying on passive absorption Enable intracellular delivery bypassing conventional absorption pathways entirely Schmitt et al
However, when ROS production exceeds the bodys ability to clear them, oxidative stress ensues
From sorting endosomes to exocytosis: association of Rab4 and Rab11 GTPases with the Fc receptor, FcRn, during recycling
