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7.3.1 Longitudinal studies on germline epigenetic inheritance 7.3.1.1 Multi-generational human cohort studies Prospective three-generation cohort studies are essential for tracking persistent DNA methylation, histone modification, and non-coding RNA changes in response to EED exposure across human generations ( 7.3.1.2 Sperm epigenome-fertility outcome integration Integration of comprehensive sperm epigenome sequencing with detailed fertility outcome data could definitively clarify the heritability of reproductive impairments ( 7.3.2 Functional validation of epigenetic modifications 7.3.2.1 CRISPR-based epigenetic editing CRISPR/dCas9-mediated epigenetic editing provides powerful tools to reverse or confirm EED-induced DNA methylation changes ( 7.3.2.2 In vitro model system development Current efforts to develop organoid models of human testicular tissue remain in early experimental phases, with significant technical challenges limiting their immediate applicability to EED research

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Neuroendocrine experiments use pituitary cell cultures (GH3, AtT-20) and primary pituitary tissue explants to measure peptide release in response to DSIP