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Beginning on day 112 1 postbreeding, 20 pregnant ewes were housed in individual pens at the Livestock Laboratory at the University of Wisconsin-Madison and maintained at a constant temperature at 18 C and a 14/10 h light/dark cycle

Because of its slow action and high post-treatment recurrence rate, ursodeoxycholic acid is not widely recommended, and its use is generally reserved for those who cannot undergo surgery (Portincasa 2012

Catalano, A

.,McKenzieCGJ,KoserU,LewisLE,BainJM,Mora-MontesHM,BarkerRN,GowNARErwigLPContribution of Candida albicans cell wall components to recognition by and escape from murine macrophages.2010;78:16501658 55

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The membranes were blocked with 5% bovine serum albumin (BSA) or 5% dry skimmed milk in Tris-buffered saline containing 0.05% (v/v) Tween-20 (TBST) for 2 h and incubated with the appropriate primary antibody in blocking buffer for 2 h at room temperature