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The selected segments were then analyzed for protein expression using 6 NanoString modules (MAPK, cell death, PI3K-AKT, immune cell typing, immune activation status, IO Drug Target) in addition to immune cell profiling core and controls
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Subsequently, 0.1 mM IPTG was added to the transfected cells, which was then cultured with 1 L LB medium for 16 h at 27 C
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