gst glutathione Glutathione, Reduced 8 and 22ml respectively Glutathione S-transferase - Wikipedia

Ang(3-7): Angiotensin II(3-7)

The cold buffer A was added to the tissue, which was manually homogenized using a motorized pestle mixer

Many drugs enter the BBB primarily through the transcellular pathway, so we can determine the physiological functional integrity of the in vitro model by immunofluorescence techniques to detect the amount of several membrane inward and outward transport proteins, such as GLUT-1, the transferrin receptor or P-glycoprotein[208]

doi: 10.1016/0959-8049(95)00145-9

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Some protocols use 5 days on / 2 days off this is optional, not required