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Briefly, cell pellets or tissues were homogenized in 1 ml TRIzol Reagent (Ambion, Life Technologies, CA), total RNAs were separated from other cell contents by sequential chloroform and isopropanol precipitation

AlqradMAIEl-AgamyDSIbrahimSRMSirwiAAbdallahHMAbdel-SattarEet al

Until the discovery of ferroptosis, it was unclear whether a GSH deficiency could be closely associated with a specific type of cell death, although the association of decreased GSH levels with oxidative stress-related cell death is implied in many diseases [28]

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When they are open, tight junctions allow material to pass between cells
